Fig 1: Expression of FoxO1 and UCN3 in nondiabetic elderly individuals. (A, C) Immunofluorescence images of pancreatic sections are shown with FoxO1 or UCN3 (red), insulin (green) and Dapi (blue) in young, middle-aged, and elderly groups. (B) Quantitative analysis of subcellular localization of FoxO1 in young, middle-aged, and elderly groups; correlation of quantified nuclear FoxO1 expression with age. (D) Quantification analysis of UCN3 intensity adjusted with islet area in young, middle-aged, and elderly groups; correlation of quantified UCN3 intensity with age. Scale bars, 20 µm. Data present means ± SD. The r of Pearson correlation and P values are shown in each panel. n = 4, *P < .05.
Fig 2: Activation of GCK induced increased expression of ATF4 and UCN3. (A, B) Representative images of immunofluorescence staining for ATF4 (A) or UCN3 (B) (red in merged channels and grayscale in split channel) and INSULIN (green) in individuals with high GCK expression (No.77, GCK: 63.3[a.u.]) and low GCK expression (No.23, GCK: 33.1[a.u.]). Scale bars, 20 µm. (C, D) Linear regression analysis was performed to detect the relationship between GCK and ATF4 (C) or UCN3 (D) expression in T2D. The black circles represent the individuals of wT2D, and the red circles represent uT2D. (E) Multivariate correlation analysis between clinical parameters (age, BMI, and FBG) and gene expressions (GCK, XBP1s, ATF4, and UCN3) in T2D. ATF4, activating transcription factor; BMI, body mass index; FBG, fasting blood glucose; GCK, glucokinase; UCN3, urocortin-3; uT2D, uncontrolled type 2 diabetes; wT2D, well-controlled type 2 diabetes; XBP1s, spliced X-box binding protein 1.
Supplier Page from MilliporeSigma for Anti-UCN3 antibody produced in rabbit